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The MAP96 is offered in two configurations that share a common mechanical envelope, communication interface, and control architecture. Both models use 9 mm channel spacing aligned to standard 96-well SBS plates, capacitive pressure-based liquid level detection (pLLD), and are validated for 1,000,000 dispense cycles under normal operating conditions. The MAP96-300 is optimized for low-to-mid volume work — ELISA dispensing, qPCR setup, and clinical sample aliquoting — with the lowest residual volume in the series at 2.5 nL. The MAP96-1000 extends the upper volume range to 1000 μL and delivers higher throughput, with an aspirate speed of 1,150 μL/s (74% faster than the MAP96-300).
| Parameter | MAP96-300 | MAP96-1000 |
|---|---|---|
| Model | MAP96-300 | MAP96-1000 |
| Maximum volume | 300 μL | 1000 μL |
| Liquid level detection | pLLD (capacitive) | pLLD (capacitive) |
| Channel pitch | 9 mm | 9 mm |
| Module weight | 4,500 g | 5,500 g |
| Maximum aspirate/dispense speed | 660 μL/s | 1,150 μL/s |
| Minimum residual volume | 2.5 nL | 4.8 nL |
| Operating temperature | +15 °C to +35 °C | +15 °C to +35 °C |
| Power input | 24 V DC (±5%) | 24 V DC (±5%) |
| Validated service life | 1,000,000 cycles | 1,000,000 cycles |
| Communication | CAN bus, RS485 | CAN bus, RS485 |
Specifications measured at +20 °C ambient, deionized water, with calibrated filter tips. Contact Keyto for the latest engineering datasheet.
Both MAP96 models reach 0.75% CV at their full working volume — 300 μL for the MAP96-300 and 1000 μL for the MAP96-1000 — representing the best precision available in the series. Performance was validated across the full operating range using filter tips of matched volume class. The data below shows single-dispense performance, the most relevant pattern for diagnostic and assay applications where each transfer is metered independently.
| Tip type | Volume (μL) | Mode | CV (%) | Accuracy A (%) |
|---|---|---|---|---|
| 20F filter tip | 1 | Single | 5.00 | 8.00 |
| 50F filter tip | 3 | Single | 8.00 | 8.00 |
| 200F filter tip | 10 | Single | 4.00 | 8.00 |
| 1000F filter tip | 300 | Single | 0.75 | 2.00 |
| Tip type | Volume (μL) | Mode | CV (%) | Accuracy A (%) |
|---|---|---|---|---|
| 20F filter tip | 5 | Single | 5.00 | 5.00 |
| 200F filter tip | 10 | Single | 5.00 | 5.00 |
| 1000F filter tip | 100 | Single | 1.00 | 2.00 |
| 1000F filter tip | 1000 | Single | 0.75 | 1.00 |
CV = coefficient of variation across 96 channels per dispense. A = inaccuracy relative to target volume. Test method: gravimetric verification with deionized water at +20 °C, calibrated balance.
Two patterns emerge from the test data. First, both modules reach their best precision at full working volume — 0.75% CV at 300 μL for the MAP96-300 and 0.75% CV at 1000 μL for the MAP96-1000 — which means OEM methods should match tip class to dispense volume rather than relying on a single tip across the full protocol. Second, the MAP96-1000 holds CV at or below 5.00% across every tested condition, while the MAP96-300 shows wider variation at the lower end of its range (8.00% CV at 3 μL with a 50F tip). For applications that require consistent precision across mixed volumes within a single run, the MAP96-1000 provides a more uniform performance envelope.
Choose the MAP96-300 when your application stays below 300 μL and residual volume is critical — typical of ELISA dispensing, qPCR setup, immunoassay reagent transfer, and clinical sample aliquoting where high-cost reagents make 2.5 nL residual a direct economic advantage. Choose the MAP96-1000 when the workflow includes reagent transfer above 300 μL, when speed is a throughput driver, or when consistent precision across mixed volumes within a single run matters — typical of NGS library preparation, bead-based normalization, and HTS dilution steps.
The MAP96 is qualified for three integration patterns that account for the majority of OEM design wins in the platform's target markets: next-generation sequencing library construction, high-throughput screening for drug discovery, and ELISA-based quality control in clinical diagnostics. Each workflow places different demands on volume range, residual, and throughput — and the MAP96 platform addresses each with model-specific configurations.
The MAP96-1000 is the recommended model for NGS library construction because library prep protocols routinely require transfers above 300 μL — including bead-based cleanup steps, ethanol washes, and elution volumes — which exceed the MAP96-300's volume ceiling. The MAP96-1000's 1,150 μL/s aspirate speed also compresses the duration of larger-volume reagent and buffer transfers, reducing total protocol time on automated workstations. The module's consistent CV ≤ 5.00% across all tested tip classes is important for library normalization, where channel-to-channel variation directly affects downstream sequencing balance.
Integration benefit: A single MAP96-1000 mounted on an XYZ gantry can process a 96-sample plate through a full NGS library prep workflow without operator intervention, releasing skilled FTE for downstream analysis.
For HTS compound screening campaigns, the MAP96-1000's 1,150 μL/s aspirate speed translates directly to plates-per-hour throughput on intermediate dilution and reagent addition steps. Screening campaigns commonly involve 384- or 1536-well formats with sub-microliter assay transfers, but the 96-well intermediate dilution operations remain the throughput-limiting step — and this is where the MAP96-1000's speed advantage over the MAP96-300 (1,150 vs 660 μL/s) is most valuable. The module's CV ≤ 5.00% across all tested conditions supports reliable hit-calling without requiring secondary confirmation runs.
Integration benefit: When integrated with a robotic plate handler and tip washer, the MAP96-1000 supports unattended overnight or weekend screening campaigns at consistent precision across the full dilution series.
The MAP96-300 is the recommended model for ELISA and quality control automation because ELISA protocols rarely exceed 300 μL per well and benefit directly from the module's 2.5 nL residual volume — the lowest in the MAP96 series. Lower residual translates to less waste of expensive ELISA conjugates and substrates, particularly across the 96-channel parallel dispense pattern where small per-channel savings compound rapidly. The pLLD capability ensures the module aspirates from the correct liquid level even when reagent volumes vary across reservoirs, and the lighter 4,500 g module weight reduces the Z-axis payload requirement on the instrument gantry.
Integration benefit: A MAP96-300 integrated with a plate washer and an absorbance reader closes the ELISA workflow loop from sample addition to optical readout, eliminating the inter-step manual handling that drives plate-to-plate CV in manual workflows.
The MAP96 is designed as a drop-in subsystem for instrument manufacturers, exposing standard CAN bus and RS485 interfaces over a 24 V DC supply. The module's command set covers aspirate, dispense, tip pickup, tip eject, and pLLD level sense.
This specification guide is published by Keyto Fluid Technology Co., Ltd., the manufacturer of the MAP96 pipetting module. All performance data presented here is sourced from Keyto's internal engineering validation under the test conditions noted in each table. Specifications are reviewed by the Keyto product engineering team and updated as the product evolves.
Keyto Fluid Technology Co., Ltd. designs and manufactures precision fluid control components — including miniature pumps, solenoid valves, microfluidic chips, syringe pumps, bonded manifolds, and 96-channel pipetting modules — for medical device, IVD, laboratory, and industrial OEM customers in Europe, North America, and Asia. Keyto components are integrated into automated diagnostic analyzers, NGS workstations, and laboratory automation platforms deployed across these markets.
All accuracy and CV figures published in this guide were measured by Keyto's engineering team using gravimetric verification with deionized water at +20 °C ambient, on a calibrated balance, using filter tips of the volume class indicated in each row. Test methodology follows the standard practice for multi-channel pipetting module validation. Application-specific performance may vary depending on reagent viscosity, temperature, tip seal condition, and integration parameters; OEM customers receive a detailed validation report alongside evaluation units.
For technical questions about MAP96 integration, performance validation, or sample evaluation, contact the Keyto applications team at en.keyto.com/contact.
